At Cisbio we provide microplates that help you discover better. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. PROTOCOL AT A GLANCE. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. 96-well culture plateFOR HTRF CERTIFIED READER. Discover our high performance reader. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. e. Typical applications The HTRF cartridge provides optimal performance for key applications. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. $352. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. If not, our scientific team will help you choose the instrument that best suits your needs and budget. It accepts all types of microtitration plate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Reader. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For more information about HTRF. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. Choosing the right microplate reader ensures you’ll get an optimal readout. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. • HTRF®-Certified Reader. FOR HTRF CERTIFIED. 55 µL anti-cAMP-Cryptate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Alpha Technology: The VICTOR Nivo system. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate Reader Requirement. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. For more information about HTRF. Make sure the setup for Tb3+ Cryptate is used. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. + 4 µL HTRF ® pre-mixed antibo dies 2. Plate 25 μL of cells in. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. FOR HTRF CERTIFIED READER . FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more ® information about HTRF compatible readers and for set-up recommendations. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. For a list of HTRF-compatible readers and set-up recommendations, please visit . HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. Microplate readers are only certified if they. For more information. Customer Transformation Hub. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. Download Setup. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. FOR HTRF CERTIFIED READER. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. The plate is set up for reading and images are acquired according to specified parameters. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Read & analyse. Make sure the setup for Eu3+ Cryptate is used. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. • HTRF®-Certified Reader. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. The VICTOR Nivo is a HTRF certified microplate reader. Read & analyse. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. FOR HTRF CERTIFIED READER. 4 mg/ml. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. This protocol. pre+ 5 µL HTRFcompounds Activation Buffer. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Read & analyse. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. Figure 1: Principle of HTRF Cortisol competitive assay. + 4 µL HTRF pre-mixed antibodies. For a list of HTRF-compatible readers and set-up recommendations, please visit . Read & analyse. Make sure the setup for Eu Cryptate is used. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. HTRF reagent concentrations have been set for optimal assay performances. 5 EXAMPLE OF MAP PLATE . We would like to show you a description here but the site won’t allow us. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Fluorescence intensity – Fluorescence intensity is one of the most. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. For more information about HTRF. Plate 25 μL of cells in. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Read & analyse. Certified for use with Alpha, LANCE ® or DELFIA ®. SpectraMax i3/i3x and Paradigm readers can be equipped with. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 96-well culture plateHTRF certified . Quantify up to 10 analytes per well. The FLUOstar Omega is the ideal plate reader for life science applications. 96-well or 384-well small volume (SV) detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. As shown in Fig. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. NC1286225. → Cover the plate with a plate sealer. Read & analyse. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Both Microplate Optimization and Read Height Adjustment were. For more information about HTRF. For more. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Make sure the setup for Tb Cryptate is used. The plate was covered and incubated for one hour at room temperature. Conclusion. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. EX: HTRF Emission range. The signals are produced by, converted by, or transmitted through the. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Read & analyse. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. 1. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. FOR HTRF CERTIFIED READER. culture plate. + 4 µL HTRF pre-mixed antibodies . 5 hours before 3 more washing steps. 8 µL cells. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. First the plate is coated with a capture antibody. FOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Even if not listed, some. As shown in Fig. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. S. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. FOR HTRF CERTIFIED READER. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in. Unlike standard ELISA plate readers, MSD. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Customer Transformation Hub. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Step 1 Step 2 Step 3 Step 4 Step 5. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. At that stage, the analyte is sandwiched between the 2 antibodies. No washing steps are required. $1,024. For more information about HTRF. Make sure the setup for Eu3+ Cryptate is used. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. To perform the assay, use white plate only. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). The controls are equivalent to the upper and lower limit of. Manufacturer. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Incubate 4h at room temperature. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. This protocol, HTS designed, allows miniaturization while. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. Specialized filters allow for IR fluorescence. Even if not listed, some readers may be compatible with HTRF technology. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. For more information about HTRF. Plus . -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. VICTOR® Nivo™ multimode plate reader 2 | For research use only. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF is compatible with a large range of available microplate readers. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. • HTRF®-Certified Reader. Remove the plate sealer and read on an HTRF® compatible reader . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. • HTRF®-Certified Reader **. 0. For more information. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate reader. best fluorescence polarization plate reader on the market. Plate 25 μL of cells in. For more. FOR HTRF CERTIFIED READER. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. Page 30 4. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. It features a modular design that allows users to add or change detection cartridges according to their needs. The mean and standard deviation. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about HTRF. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. 6% for 1,408 wells and a Z’ value of 0. (donor) of compounds are measured on an HTRF-compatible reader. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Make sure the setup for Tb Cryptate is used. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. FOR HTRF CERTIFIED READER For more. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). High Speed Monochromator offers a typical wavelength accuracy of <0. This protocol, HTS designed, allows miniaturization while. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. Make sure the setup for Eu Cryptate is used. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. These plates can be read on all HTRF certified readers, thus making their implementation in. They can be used in a no-transfer. These plates can be read on all HTRF certified readers, thus making their implementation in. Make sure the setup for Tb Cryptate is used. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. and Canaa -8-963-4567. It continues to deliver optimal results. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. 68. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit . • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Optimized instrument settings for the HTRF camps HiRange assay. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Incubate at room temperature. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. FOR HTRF CERTIFIED READER. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. This protocol gives the cells viability and confluence to be monitored. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. FOR HTRF CERTIFIED READER. The assay is then ready to be read on an HTRF compatible or certified microplate reader. Plate Type and Read Area should be selected based on the microplate used and wells to be read. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. The VICTOR Nivo is a HTRF certified microplate reader. Results for HTRF Reader Control Kit with black and white microplates. COMPONENTS ; STORAGE . Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. Phospho-Rb (Ser780) two-plate assay protocol. VICTOR Nivo Multimode Plate Reader. The ratio for each well must be calculated individually. CLARIOstar is equipped with a specific optical device which enables the measurement of. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. These plates can be read on all HTRF certified readers, thus making their implementation in. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. For more information about HTRF. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. HTRF-specific. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The optimized well design and high quality plastics provides low background and crosstalk. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate 2h at RT Transfer 16 µL supernatant. Plate 25 μL of cells in. In addition, stacker. The donor and acceptor can be used to label a variety of biomolecules, for. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. These plates can be read on all HTRF certified readers, thus making their implementation in. 10 µL Standard or Sample. HTRF Signal + 5 hours @ RT or . This protocol gives the cells viability and confluence to be monitored. HTRF certified . Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The HTRF ratio obtained represents the total binding. These plates can be read on all HTRF certified readers, thus making their implementation in. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. These plates can be read on all HTRF certified readers, thus making their implementation in. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. Lyse cells Read on an HTRF compatible reader . For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Specialized filters allow for IR fluorescence. The assay was prepared in both white and black plates and incubated >18 hours. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. Assay setup for a 384-well low-volume plate. VICTOR® Nivo™ multimode plate reader. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. * For HTRF microplate recommendations, please visit . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Transcreener® ADP2 TR-FRET assay. For more information about HTRF ®• HTRF®-Certified Reader. Read & analyse. As shown in Fig. Some vendors hold the entire market share for not only a. Remove the plate seal and read on an HTRF compatible reader. 15 / Each of 1; Qty. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. For more information. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. Partnerships for delivering turn-key solutions. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. PURCHASE SEPARATELY . Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. No washing steps are required. This protocol, HTS designed, allows miniaturization while. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Homogeneous Time-Resolved Fluorescence (HTRF) . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. But sometimes these parameters are not sufficient enough. This protocol gives the cells viability and confluence to be monitored. At Cisbio we provide microplates that help you discover better. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit. To perform the assay, use white plate only.